《植物生理学报》 2009, 45(9): 847-850

光照和温度对扇蕨孢子萌发的影响

张光飞^1,*, 翟书华², 杨慧¹, 苏文华¹

1 云南大学生态学与地植物学研究所, 昆明650091; 2 昆明学院生命科学与技术系, 昆明650031

通信作者：张光飞；E-mail: gfzhang@ynu.edu.cn；Tel: 0871-5033535

摘　要：

用组织培养法和光学显微镜技术初步研究光照和温度影响扇蕨孢子萌发的结果表明, 扇蕨孢子发芽是需光型, 具有 明显的光休眠现象。光照是孢子萌发的主要影响因子, 25 ℃下, 孢子萌发率达(85.1±5.1)%。相同温度下孢子即使在黑暗中 培养 50 d 也不能萌发, 转入光照下后萌发率可达(82.4±6.6)%。孢子在光下的最适发芽温度为 21.6~26.5 ℃, 7 d 开始萌发,6~7周完全萌发, 温度升高或下降均降低孢子萌发率。

关键词：扇蕨; 孢子萌发; 光照; 温度

收稿：2009-05-03   修定：2009-08-12

资助：国家自然科学基金(30760043)和教育部高等理工教育教学改革与实践项目(239)。

Influence of Light and Temperature on Spore Germination of Neocheiropteris palmatopedata (Baker) Christ

ZHANG Guang-Fei^1,*, ZHAI Shu-Hua², YANG Hui¹, SU Wen-Hua¹

¹Institute of Ecology and Geobotany, Yunnan University, Kunming 650091, China; ²Life Science and Technology Department, Kunming University, Kunming 650031, China

Corresponding author: ZHANG Guang-Fei; E-mail: gfzhang@ynu.edu.cn; Tel: 0871-5033535

Abstract:

The influence of light and temperature on the spore germination of Neocheiropteris palmatopedata were studied by tissue culture and light microscopy. The preliminary results showed the spore germination of N. palmatopedata depended on light and had photodormancy. Light was the major influence factor of spore germination. At temperature of 25 ℃, the spore germination percentage reached (85.1±5.1)%. At the same temperature there was no spore germination in the dark after 50 days of culture, and then the spores were transferred into light, the spore germination percentage reached (82.4±6.6)%. The optimal temperatures for germination was 21.6–26.5 ℃ in light. The spores began to germinate after 7 days and germinated completely after 6–7 weeks. The changes in temperature could reduce the spore germination percentage.

Key words: Neocheiropteris palmatopedata; spore germination; light; temperature